by Carly Karrick. Viruses play important roles in evolution and influence ecosystems and host health. However, isolating and studying them can be difficult. In lieu of using resource-intensive methods to concentrate viruses into a “virome,” bulk sequencing methods include data from all biological entities present in a sample. In this tutorial, we explore an approach to mine viral sequences from publicly available bulk RNA-Seq data. The output from this analysis paves the way for future statistical analyses comparing viral communities in different contexts. This approach can be applied to other datasets, including studies of human health.
